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Image Search Results
Journal: International journal of biological sciences
Article Title: Specific Pyruvate Kinase M2 Inhibitor, Compound 3K, Induces Autophagic Cell Death through Disruption of the Glycolysis Pathway in Ovarian Cancer Cells.
doi: 10.7150/ijbs.59855
Figure Lengend Snippet: Figure 7. Changes in the expression of various proteins in signaling pathways following PKM2 inhibition by compound 3K for 24 h. (A) The effect of PKM2 inhibition on signaling pathways in SK-OV-3 cells. Cells were treated with compound 3K, and western blot analysis was conducted to determine the expression levels of various signaling pathway proteins. (B) The band intensity of Akt, p-Akt, AMPK, p-AMPK, mTOR, p-mTOR, p70S6K, and p-p70S6K were calculated and expressed in the graph as a ratio of β-actin. The values represent the mean ± SD. *p < 0.05 and **p < 0.01.
Article Snippet: Primary antibodies against PKM2, PKM1, Cyclin B1, p-Cdc2, Cdc2, Bax, Bcl-2, caspase 3, Beclin-1, LC3B, Atg5, Atg7, p62, MMP-2, MMP-9, TIMP-2, p-AKT, AKT, p-AMPK, AMPK, p-mTOR,
Techniques: Expressing, Protein-Protein interactions, Inhibition, Western Blot
Journal: International journal of biological sciences
Article Title: Specific Pyruvate Kinase M2 Inhibitor, Compound 3K, Induces Autophagic Cell Death through Disruption of the Glycolysis Pathway in Ovarian Cancer Cells.
doi: 10.7150/ijbs.59855
Figure Lengend Snippet: Figure 10. Schematic diagram illustrating potential pathways associated with cell death in compound 3K-treated SK-OV-3 cells. Compound 3K-induced cell death is associated with the Akt/AMPK/mTOR signaling pathway. Compound 3K induces phosphorylation of AMPK at Thr172 and reduces the phosphorylation of Akt. These molecular changes cause the suppression of mTOR signaling, thereby regulating autophagy and apoptosis.
Article Snippet: Primary antibodies against PKM2, PKM1, Cyclin B1, p-Cdc2, Cdc2, Bax, Bcl-2, caspase 3, Beclin-1, LC3B, Atg5, Atg7, p62, MMP-2, MMP-9, TIMP-2, p-AKT, AKT, p-AMPK, AMPK, p-mTOR,
Techniques: Phospho-proteomics